Plasmid

Part:BBa_J100270:Design

Designed by: Owen Koucky   Group: Campbell M Lab   (2016-06-17)


SP-T7Specific_L2-48.3 (Carlson et al. 2014)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5475
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2016
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5475
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5475
    Illegal NgoMIV site found at 8437
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014


Source

"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014

References